CellLine¶
lamindb provides access to the following public protein ontologies through bionty:
Here we show how to access and search CellLine ontologies to standardize new data.
import bionty as bt
import pandas as pd
💡 connected lamindb: testuser1/test-public-ontologies
PublicOntology objects¶
Let us create a public ontology accessor with .public method, which chooses a default public ontology source from PublicSource.
It’s a PublicOntology object, which you can think about as a public registry:
celllines = bt.CellLine.public(organism="all")
celllines
PublicOntology
Entity: CellLine
Organism: all
Source: clo, 2022-03-21
#terms: 39037
As for registries, you can export the ontology as a DataFrame:
df = celllines.df()
df.head()
| name | definition | synonyms | parents | |
|---|---|---|---|---|
| ontology_id | ||||
| CLO:0000000 | cell line cell culturing | a maintaining cell culture process that keeps ... | None | [] |
| CLO:0000001 | cell line cell | A cultured cell that is part of a cell line - ... | None | [] |
| CLO:0000002 | suspension cell line culturing | suspension cell line culturing is a cell line ... | None | [CLO:0000000] |
| CLO:0000003 | adherent cell line culturing | adherent cell line culturing is a cell line cu... | None | [CLO:0000000] |
| CLO:0000004 | cell line cell modification | a material processing that modifies an existin... | None | [] |
Unlike registries, you can also export it as a Pronto object via public.ontology.
Look up terms¶
As for registries, terms can be looked up with auto-complete:
lookup = celllines.lookup()
The . accessor provides normalized terms (lower case, only contains alphanumeric characters and underscores):
lookup.hek293
CellLine(ontology_id='CLO:0001230', name='HEK293', definition=None, synonyms='293|HEK-293|293 HEK|293 cell|Human Embryonic Kidney 293|HEK 293', parents=array(['CLO:0037236'], dtype=object))
To look up the exact original strings, convert the lookup object to dict and use the [] accessor:
lookup_dict = lookup.dict()
lookup_dict["HEK293"]
CellLine(ontology_id='CLO:0001230', name='HEK293', definition=None, synonyms='293|HEK-293|293 HEK|293 cell|Human Embryonic Kidney 293|HEK 293', parents=array(['CLO:0037236'], dtype=object))
By default, the name field is used to generate lookup keys. You can specify another field to look up:
lookup = celllines.lookup(celllines.ontology_id)
lookup.clo_0000469
CellLine(ontology_id='CLO:0000469', name='immortal cat mixed endoderm/mesoderm-derived structure-derived cell line cell', definition='An immortal mixed endoderm/mesoderm-derived structure-derived cell line cell that derives from cat.', synonyms=None, parents=array(['CLO:0000213'], dtype=object))
Search terms¶
Search behaves in the same way as it does for registries:
celllines.search("hek293").head(3)
| ontology_id | definition | synonyms | parents | __ratio__ | |
|---|---|---|---|---|---|
| name | |||||
| 293-derived cell | CLO:0037237 | None | 293|HEK-293|293 HEK|HEK293|HEK 293 | [CLO:0037236] | 100.000000 |
| HEK293H cell | CLO:0037346 | HEK293 cells expressing E1A adenovirus gene, e... | HEK293H | [CLO:0037237] | 92.307692 |
| HEK293T cell | CLO:0037372 | None | 293T|293-T|HEK 293 T|HEK-293T|HEK293T|293tsA16... | [CLO:0037237] | 92.307692 |
By default, search also covers synonyms:
celllines.search("Human Embryonic Kidney 293").head(3)
| ontology_id | definition | synonyms | parents | __ratio__ | |
|---|---|---|---|---|---|
| name | |||||
| HEK293 | CLO:0001230 | None | 293|HEK-293|293 HEK|293 cell|Human Embryonic K... | [CLO:0037236] | 100.000000 |
| HEK293T cell | CLO:0037372 | None | 293T|293-T|HEK 293 T|HEK-293T|HEK293T|293tsA16... | [CLO:0037237] | 98.113208 |
| HEK293T-derived cell | CLO:0037373 | None | Human Embryonic Kidney 293T-derived cell | [CLO:0037237] | 78.787879 |
You can turn this off synonym by passing synonyms_field=None:
celllines.search("Human Embryonic Kidney 293", synonyms_field=None).head(3)
| ontology_id | definition | synonyms | parents | __ratio__ | |
|---|---|---|---|---|---|
| name | |||||
| human embryonic stem cell line cell | CLO:0037280 | An embryonic stem cell line cell that is dervi... | hESC line cell|hES cell line cell|hESC | [CLO:0037279] | 65.573770 |
| human stem cell line cell | CLO:0037335 | A stem cell line cell that is derived from human. | None | [CLO:0037224] | 54.901961 |
| immortal extraembryonic cell line cell | CLO:0000145 | a immortal animal cell line that is derived fr... | None | [CLO:0000166, CLO:0000128] | 53.125000 |
Search another field (default is .name):
celllines.search(
"suspension cell line",
field=celllines.definition,
).head()
| ontology_id | name | synonyms | parents | __ratio__ | |
|---|---|---|---|---|---|
| definition | |||||
| a cell line that requires suspension cell cuturing. | CLO:0000026 | suspension cell line cell | suspension cell line | [CLO:0000001] | 100.000000 |
| A cell line that is expected to be capable of indefinite propagation in an vitro culture. | CLO:0009828 | immortal cell line | permanent cell line | [CLO:0000031] | 71.794872 |
| A cell line is able to support only a limited number of passages in vitro.|A mortal cell line is not capable of supporting indefinite propagation in vitro, being limited by entry of its cells into senescence. | CLO:0009829 | mortal cell line | primary cell line|finite cell line | [CLO:0000031] | 66.666667 |
| A cell line comprised of cells which can all be traced back to a single ancestor cell, and which therefore can be treated as identical. This can be used to study just the progeny of single cells. | CLO:0000177 | cloned cell line | clonal cell line | [CLO:0000031] | 66.666667 |
| A stem cell line cell that is dervied from an embryotic stem cell, a pluripotent stem cell derived from the inner cell mass of a blastocyst, an early-stage perimplantation embryo. | CLO:0037279 | embryonic stem cell line cell | ES cell line cell | [CLO:0037224] | 64.864865 |
Standardize CellLine identifiers¶
Let us generate a DataFrame that stores a number of CellLine identifiers, some of which corrupted:
df_orig = pd.DataFrame(
index=[
"253D cell",
"HEK293",
"2C1H7 cell",
"283TAg cell",
"This cellline does not exist",
]
)
df_orig
| 253D cell |
|---|
| HEK293 |
| 2C1H7 cell |
| 283TAg cell |
| This cellline does not exist |
We can check whether any of our values are validated against the ontology reference:
validated = celllines.validate(df_orig.index, celllines.name)
df_orig.index[~validated]
✅ 4 terms (80.00%) are validated
❗ 1 term (20.00%) is not validated: This cellline does not exist
Index(['This cellline does not exist'], dtype='object')
Ontology source versions¶
For any given entity, we can choose from a number of versions:
bt.PublicSource.filter(entity="CellLine").df()
| uid | entity | organism | currently_used | source | source_name | version | url | md5 | source_website | run_id | created_by_id | updated_at | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| id | |||||||||||||
| 28 | 6cbC | CellLine | all | True | clo | Cell Line Ontology | 2022-03-21 | https://data.bioontology.org/ontologies/CLO/su... | ea58a1010b7e745702a8397a526b3a33 | https://bioportal.bioontology.org/ontologies/CLO | None | 1 | 2024-05-29 09:56:16.486097+00:00 |
When instantiating a Bionty object, we can choose a source or version:
public_source = bt.PublicSource.filter(
source="clo", version="2022-03-21", organism="all"
).one()
celllines= bt.CellLine.public(public_source=public_source)
celllines
PublicOntology
Entity: CellLine
Organism: all
Source: clo, 2022-03-21
#terms: 39037
The currently used ontologies can be displayed using:
bt.PublicSource.filter(currently_used=True).df()
Show code cell output
| uid | entity | organism | currently_used | source | source_name | version | url | md5 | source_website | run_id | created_by_id | updated_at | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| id | |||||||||||||
| 1 | 5Dlc | Organism | vertebrates | True | ensembl | Ensembl | release-112 | https://ftp.ensembl.org/pub/release-112/specie... | 0ec37e77f4bc2d0b0b47c6c62b9f122d | https://www.ensembl.org | None | 1 | 2024-05-29 09:56:16.483555+00:00 |
| 6 | 2Jzh | Organism | bacteria | True | ensembl | Ensembl | release-57 | https://ftp.ensemblgenomes.ebi.ac.uk/pub/bacte... | ee28510ed5586ea7ab4495717c96efc8 | https://www.ensembl.org | None | 1 | 2024-05-29 09:56:16.484046+00:00 |
| 7 | 1kdI | Organism | fungi | True | ensembl | Ensembl | release-57 | http://ftp.ensemblgenomes.org/pub/fungi/releas... | dbcde58f4396ab8b2480f7fe9f83df8a | https://www.ensembl.org | None | 1 | 2024-05-29 09:56:16.484138+00:00 |
| 8 | 2mIM | Organism | metazoa | True | ensembl | Ensembl | release-57 | http://ftp.ensemblgenomes.org/pub/metazoa/rele... | 424636a574fec078a61cbdddb05f9132 | https://www.ensembl.org | None | 1 | 2024-05-29 09:56:16.484233+00:00 |
| 9 | 2XQ6 | Organism | plants | True | ensembl | Ensembl | release-57 | https://ftp.ensemblgenomes.ebi.ac.uk/pub/plant... | eadaa1f3e527e4c3940c90c7fa5c8bf4 | https://www.ensembl.org | None | 1 | 2024-05-29 09:56:16.484333+00:00 |
| 10 | 1Vzs | Organism | all | True | ncbitaxon | NCBItaxon Ontology | 2023-06-20 | s3://bionty-assets/df_all__ncbitaxon__2023-06-... | 00d97ba65627f1cd65636d2df22ea76c | https://github.com/obophenotype/ncbitaxon | None | 1 | 2024-05-29 09:56:16.484429+00:00 |
| 11 | 1hx4 | Gene | human | True | ensembl | Ensembl | release-112 | s3://bionty-assets/df_human__ensembl__release-... | 4ccda4d88720a326737376c534e8446b | https://www.ensembl.org | None | 1 | 2024-05-29 09:56:16.484524+00:00 |
| 15 | 76FX | Gene | mouse | True | ensembl | Ensembl | release-112 | s3://bionty-assets/df_mouse__ensembl__release-... | 519cf7b8acc3c948274f66f3155a3210 | https://www.ensembl.org | None | 1 | 2024-05-29 09:56:16.484895+00:00 |
| 19 | 7LW6 | Gene | saccharomyces cerevisiae | True | ensembl | Ensembl | release-112 | s3://bionty-assets/df_saccharomyces cerevisiae... | 11775126b101233525a0a9e2dd64edae | https://www.ensembl.org | None | 1 | 2024-05-29 09:56:16.485271+00:00 |
| 22 | 7llW | Protein | human | True | uniprot | Uniprot | 2023-03 | s3://bionty-assets/df_human__uniprot__2023-03_... | 1c46e85c6faf5eff3de5b4e1e4edc4d3 | https://www.uniprot.org | None | 1 | 2024-05-29 09:56:16.485547+00:00 |
| 24 | 5U7J | Protein | mouse | True | uniprot | Uniprot | 2023-03 | s3://bionty-assets/df_mouse__uniprot__2023-03_... | 9d5e9a8225011d3218e10f9bbb96a46c | https://www.uniprot.org | None | 1 | 2024-05-29 09:56:16.485730+00:00 |
| 26 | 5nkB | CellMarker | human | True | cellmarker | CellMarker | 2.0 | s3://bionty-assets/human_cellmarker_2.0_CellMa... | d565d4a542a5c7e7a06255975358e4f4 | http://bio-bigdata.hrbmu.edu.cn/CellMarker | None | 1 | 2024-05-29 09:56:16.485913+00:00 |
| 27 | 6AFz | CellMarker | mouse | True | cellmarker | CellMarker | 2.0 | s3://bionty-assets/mouse_cellmarker_2.0_CellMa... | 189586732c63be949e40dfa6a3636105 | http://bio-bigdata.hrbmu.edu.cn/CellMarker | None | 1 | 2024-05-29 09:56:16.486005+00:00 |
| 28 | 6cbC | CellLine | all | True | clo | Cell Line Ontology | 2022-03-21 | https://data.bioontology.org/ontologies/CLO/su... | ea58a1010b7e745702a8397a526b3a33 | https://bioportal.bioontology.org/ontologies/CLO | None | 1 | 2024-05-29 09:56:16.486097+00:00 |
| 29 | 3DeN | CellType | all | True | cl | Cell Ontology | 2024-02-13 | http://purl.obolibrary.org/obo/cl/releases/202... | https://obophenotype.github.io/cell-ontology | None | 1 | 2024-05-29 09:56:16.486189+00:00 | |
| 34 | 1AyH | Tissue | all | True | uberon | Uberon multi-species anatomy ontology | 2024-02-20 | http://purl.obolibrary.org/obo/uberon/releases... | 2048667b5fdf93192384bdf53cafba18 | http://obophenotype.github.io/uberon | None | 1 | 2024-05-29 09:56:16.486674+00:00 |
| 39 | LoCG | Disease | all | True | mondo | Mondo Disease Ontology | 2024-02-06 | http://purl.obolibrary.org/obo/mondo/releases/... | 78914fa236773c5ea6605f7570df6245 | https://mondo.monarchinitiative.org | None | 1 | 2024-05-29 09:56:16.487135+00:00 |
| 44 | 2mou | Disease | human | True | doid | Human Disease Ontology | 2024-01-31 | http://purl.obolibrary.org/obo/doid/releases/2... | b36c15a4610757094f8db64b78ae2693 | https://disease-ontology.org | None | 1 | 2024-05-29 09:56:16.487603+00:00 |
| 51 | 4usY | ExperimentalFactor | all | True | efo | The Experimental Factor Ontology | 3.63.0 | http://www.ebi.ac.uk/efo/releases/v3.63.0/efo.owl | 603e6f6981d53d501c5921aa3940b095 | https://bioportal.bioontology.org/ontologies/EFO | None | 1 | 2024-05-29 09:56:16.488253+00:00 |
| 54 | 2WLc | Phenotype | human | True | hp | Human Phenotype Ontology | 2024-03-06 | https://github.com/obophenotype/human-phenotyp... | 36b0d00c24a68edb9131707bc146a4c7 | https://hpo.jax.org | None | 1 | 2024-05-29 09:56:16.488529+00:00 |
| 58 | 6zE1 | Phenotype | mammalian | True | mp | Mammalian Phenotype Ontology | 2024-02-07 | https://github.com/mgijax/mammalian-phenotype-... | 31c27ed2c7d5774f8b20a77e4e1fd278 | https://github.com/mgijax/mammalian-phenotype-... | None | 1 | 2024-05-29 09:56:16.488896+00:00 |
| 60 | 7EnA | Phenotype | zebrafish | True | zp | Zebrafish Phenotype Ontology | 2024-01-22 | https://github.com/obophenotype/zebrafish-phen... | 01600a5d392419b27fc567362d4cfff8 | https://github.com/obophenotype/zebrafish-phen... | None | 1 | 2024-05-29 09:56:16.489080+00:00 |
| 63 | 55lY | Phenotype | all | True | pato | Phenotype And Trait Ontology | 2023-05-18 | http://purl.obolibrary.org/obo/pato/releases/2... | bd472f4971492109493d4ad8a779a8dd | https://github.com/pato-ontology/pato | None | 1 | 2024-05-29 09:56:16.489354+00:00 |
| 64 | 48aa | Pathway | all | True | go | Gene Ontology | 2023-05-10 | https://data.bioontology.org/ontologies/GO/sub... | e9845499eadaef2418f464cd7e9ac92e | http://geneontology.org | None | 1 | 2024-05-29 09:56:16.489445+00:00 |
| 67 | 3rm9 | BFXPipeline | all | True | lamin | Bioinformatics Pipeline | 1.0.0 | s3://bionty-assets/bfxpipelines.json | a7eff57a256994692fba46e0199ffc94 | https://lamin.ai | None | 1 | 2024-05-29 09:56:16.489724+00:00 |
| 68 | 5alK | Drug | all | True | dron | Drug Ontology | 2024-03-02 | https://data.bioontology.org/ontologies/DRON/s... | 84138459de4f65034e979f4e46783747 | https://bioportal.bioontology.org/ontologies/DRON | None | 1 | 2024-05-29 09:56:16.489824+00:00 |
| 70 | 7CRn | DevelopmentalStage | human | True | hsapdv | Human Developmental Stages | 2020-03-10 | http://aber-owl.net/media/ontologies/HSAPDV/11... | 52181d59df84578ed69214a5cb614036 | https://github.com/obophenotype/developmental-... | None | 1 | 2024-05-29 09:56:16.490012+00:00 |
| 71 | 16tR | DevelopmentalStage | mouse | True | mmusdv | Mouse Developmental Stages | 2020-03-10 | http://aber-owl.net/media/ontologies/MMUSDV/9/... | 5bef72395d853c7f65450e6c2a1fc653 | https://github.com/obophenotype/developmental-... | None | 1 | 2024-05-29 09:56:16.490106+00:00 |
| 72 | 3Tlc | Ethnicity | human | True | hancestro | Human Ancestry Ontology | 3.0 | https://github.com/EBISPOT/hancestro/raw/3.0/h... | 76dd9efda9c2abd4bc32fc57c0b755dd | https://github.com/EBISPOT/hancestro | None | 1 | 2024-05-29 09:56:16.492428+00:00 |
| 73 | 5JnV | BioSample | all | True | ncbi | NCBI BioSample attributes | 2023-09 | s3://bionty-assets/df_all__ncbi__2023-09__BioS... | 918db9bd1734b97c596c67d9654a4126 | https://www.ncbi.nlm.nih.gov/biosample/docs/at... | None | 1 | 2024-05-29 09:56:16.492535+00:00 |